Plasmid
Part:BBa_k2061003
Designed by: avi matityahu Group: iGEM16_Tel-Hai (2016-08-23)
This part contains 20 bp of target sequence in CFTR exon 11, PAM sequence (NGG) and the gRNA scaffold under the control of U6 promoter and terminator. The part was cloned in pSB1C3 between EcoRI and PstI sites. This part will serve for generating the second gRNA leading the CAS9 to the CFTR exon 11 for gene editing.
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